The Way to a Deeper Insight into the Cell

nanoFLeye (nanoFluorescenceEye) is the innovative reply on demands and needs in the field of superresolving optical imaging based on the localization microscopy technique SPDM (spectral precision distance microscopy).

nanoFLeye excels with

3D Multicolour Imaging based on SPDM

High Stability

High Flexibility

Choose up to Four Different Excitation Wavelengths Suitable to your Desired Dyes

Choose your Favoured Microscope Objective

Clear and User-Friendly Interface

Possibility of Remote Control

Opportunity of High Level Automatization such as Autofocus, Easily Programmable Measurement Sequences and Automated Data Analysis


nanoFLeye is equipped with the pioneering ReconFlex camera developed by Surface Concept GmbH making localization microscopy substantially easier and faster.


Localization microscopy in general has up to now been characterized by recording an image stack comprising tens of thousands of images and time-consuming post-processing of the data to determine the position of each molecule (reconstruction).


ReconFlex facilitates an on-the-fly data analysis for localization microscopy providing superresolved images in real-time.



It offers ultra-high flexibilty in terms of its two different modes:

Normal Camera Mode: For  Live Imaging, adjusting the Microscope and recording Imaging Stacks

Reconstruction Mode:  The Localization of the Molecules is being determined by the Camera itself

Both modes can also be applied simultaneously.


Enormous Reduction in Data Transfer, Time and Disk Space

Select Fiducial Markers prior to the Measurement to correct Possible Drifts in Real-Time

The User retains Full Control over the Algorithms used in the Reconstruction Mode as there is the Possibility of implementing User-Developed Algorithms

Provided with ReconFlex, nanoFLeye opens up a new door to real-time, easy and user-friendly superresolving localization microscopy.


Biomedical as well as material science applications due to flexible sample holder as well as bottom-up setup


Easy sample preparation due to usage of conventional fluorescent dyes

Conventional epifluorescent microscopy image of Alexa647-labeled microtubuli of HeLa-cells.

Superresolved image of the identical sample position recorded by nanoFleye. The scale bar (horizontal line bottom left) corresponds to 1µm.

Linescan (vertical line in the upper image) of the epifluorescent image.

Linescan (vertical line in the upper image) of the superresolved image.

Alexa680-labeled human platelets (HuPLTs, PF4, A680, native)

sample preparation courtesy of: Dr. M. Schmitt, LMU München

left: conventional epifluorescent microscopy image

right: superresolved image recorded by nanoFLeye

Conventional epifluorescent microscopy image of Alexa680-labeled HuPLTs. The image size is 5µm x 5µm.

Superresolved image of the identical sample position recorded by nanoFLeye.


Convential fluorescence microscopy is a versatile tool to perform functional cell biology analysis. Fluorophores are being coupled to antibodies which bind to their corresponding proteins in the cell. By analyzing the fluorescence signals in the microscopy image, one can get insight to  the distribution of the chosen proteins inside the cell.


However, in terms of nano-science and detailed insight into biological processes on a molecular level, convential fluorescence microscopy is stretched to its limit.


In an epifluorescence microscope the lateral resolution is determined by the diffraction limit, i.e. you cannot distinguish two molecules having a distance less than ~200 nm from each other. In a confocal setup it is possible to improve the resolution slightly, but not sufficient to detect single molecules.

Localization Microscopy

Spectral features are used to achieve optical isolation















In Conventional Fluorescence Microscopy the FWHM of the Point-Spread-Function (PSF) >200 nm. Signals of adjacent dyes overlap, therefore single molecules cannot be resolved


Using SPDM, randomly activated dyes are „optically isolated“, i.e. no overlap of the signal of adjacent molecules can occur


The locations of the optically isolated fluorophores are determined by the localization algorithm with a precision down to 20 nm


All localizations found in a stack of usually ten thousands of images are displayed in a single reconstructed super-resolved image



Surface Concept GmbH


Am Sägewerk 23a

55124 Mainz



phone:  +49 6131 62716 0

fax:        +49 6131 62716 29


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